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CO2 Independent medium with L-Glutamine and HI-FBS
| Reagent | Concentration | Amount |
|---|---|---|
| L-glutamine (200 mM) | 2% of medium volume | 0.26 mL |
| HI-FBS | 10% of total volume | 1.5 mL |
| CO2 Independent Medium | N/A | 13.24 mL |
| Total | N/A | 15 mL |
Store at 4°C, discard if not used within two weeks.
