REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
R848 (resiquimod)	InvivoGen	tlrl-r848
CO2 independent medium	Fisher Scientific	18045088
DMEM (Dulbecco’s modified Eagle’s medium)	Thermo Fisher Scientific	11995073
FBS (fetal bovine serum)	Thermo Fisher Scientific	26140079
L-glutamine (200 mM solution)	Thermo Fisher Scientific	25030081
NovoRinse	Agilent	872B603
NovoClean	Agilent	872B602
Hoechst 33342, trihydrochloride, trihydrate	Fisher Scientific	H3570
Alexa Fluor 488	Thermo Fisher Scientific	33077A
Deposited data
Data referenced in this paper	This paper	Mendeley Data: https://doi.org/10.17632/kgtt279fp7.2
Experimental models: Cell lines
Murine: RAW264.7 macrophages, GFP NF-kB reporter	Iain Fraser Lab Sung et al. 2014	RRID: CVCL_0493, ATCC TIB71
Software and algorithms
Fiji	Schindelin et al. 2012	RRID: SCR_002285 https://fiji.sc
CellProfiler	McQuin et al. 2018	RRID: SCR_007358 https://cellprofiler.org
Cytosurge FluidFM software	Cytosurge	https://documentation.cytosurge.com/cora/
FluidFM C3000 controller software	Nanosurf	https://www.nanosurf.com/en/software/c3000
ZEISS ZEN Pro microscope software	ZEISS	https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html
RStudio	RStudio Team 2020	RRID: SCR_000432 http://www.rstudio.com
GraphPad Prism	GraphPad Software	RRID: SCR_002798 http://graphpad.com
Mathematica	Wolfram Research	RRID: SCR_014448 https://www.wolfram.com/mathematica/
Code referenced in this paper	This paper	Mendeley Data: https://doi.org/10.17632/kgtt279fp7.2
Other
FlexAFM	Nanosurf	https://www.nanosurf.com/en/products/flexafm
FluidFM micropipette probes 2 N/m stiffness, 2 micron aperture, FlexAFM	Cytosurge	www.cytosurge.com/shop/product/fluidfm-micropipette-208#attr=187,172,52
Axio Observer 7 inverted optical microscope with live cell incubation box	ZEISS	000000-2624-360
ORCA-Flash4.0 V3 xCMOS camera	Hamamatsu	C13440-20CU-KIT
Microscopy dishes 50 mm × 7 mm	Ted Pella	14027-200
Cover glass 24 mm × 50 mm	Fisher Scientific	22-050-232
Syringe filter, 0.2 μm, PTFE	Corning	431212