Fig. 1.

MiR-7 deficiency alleviates the pathology of murine IBD and promotes epithelial regeneration. (A) Schematic representation of the establishment of murine DSS-induced IBD. MiR-7^def mice and WT mice (8–10 weeks old, n = 8) were given 2% DSS solution for 7 days and changed water to continue feeding for 3 days to induce the IBD model; the mice were sacrificed on the 10th day. The weight loss (B), the survival curve (C) of WT and miR-7^def littermates after DSS treatment were observed; Representative gross images of colon and colon length were detected and analyzed (D); The clinical scores were estimated (E); Histopathology was performed by H&E staining and mucin production measured by Alcian blue staining (×200) (F); The relative expression levels of proinflammatory cytokines IL-6, TNF-α, TGF-β, IL-10, CSF4, CCL4, CXCL3, and CX3CL1 were assessed in the colon tissue by Real-time PCR assay (G); Double immunofluorescence for Ki67 and EPCAM in the colon tissue (×200) (H); The expression of CD45, p-NF-kB and EPCAM were determined by multiplexed fluorescent immunohistochemical staining in the colon (×3, ×400) (I). The values are the means ± SD (n = 3). *P < 0.05, **P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)