Fig. 2.

MiR-7 deficiency reduces the infiltration of inflammatory cells in colon tissue in IBD. (A–B) The gene-expression profiles in the colorectal tissues of miR-7^def and WT IBD mice were performed and analyzed using RNA-seq assay. GSEA plots from RNA-seq data observed the inflammatory-related genes. (C) The proportion of CD45⁺ cells were detected and analyzed in the colon tissue by FCM; The expression of CD45 was determined by IF (D); The expression of CD4⁺ T cells, CD8⁺ T cells, CD19⁺ B cells, Gr-1⁺ cells, CD11b⁺ cells, NK1.1⁺ T cells, CD11c⁺ cells, F4/80⁺ cells and γδ⁺ T cells in CD45⁺ cells were analyzed and calculated by FCM (E–H); The expression of CD4⁺ T cells, CD8⁺ T cells, and CD11b⁺ cells were detected by Multiplexed fluorescent immunohistochemical staining (I). The values are the means ± SD (n = 3). *P < 0.05, **P < 0.01.