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. 2023 Apr 23;57:119–134. doi: 10.1016/j.jare.2023.04.011

Fig. 3.

Fig. 3

MiR-7 expression is upregulated in colonic epithelial cells of mouse colitis. (A) Schematic representation of the establishment of murine DSS-induced IBD. WT mice (8–10 weeks old, n = 8) were given 2% DSS solution for 7 days and changed water to continue feeding for 3 days to induce IBD model; MiR-7 expression was detected in the colon tissue at the indicated time points by Real-time PCR (B). (C) CD45+ inflammatory cells in colorectal tissue and CD45− colonic epithelial cells of colorectal tissue were purified by MACS, then the levels of miR-7 were detected using Real-time PCR assay. (D) The expression and localization of miR-7 in EPCAM+ IECs of colorectal tissue was verified by FISH. (E) Human colonic epithelium cells FHC cells were treated with LPS (200 ng/ml), and the expression of miR-7 at different time points (0, 6, 12, and 18 h) was detected and analyzed by Real-time PCR. (F) IECs from WT mice were treated by LPS (500 ng/ml); 48 h later, miR-7 expression was analyzed by Real-time PCR. The values are the means ± SD (n = 3). *P < 0.05, **P < 0.01.