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. 2023 Apr 23;57:119–134. doi: 10.1016/j.jare.2023.04.011

Fig. 7.

Fig. 7

MiR-7 controls epithelial immunomodulation and regeneration via EGFR in vitro. (A) The normal colonic epithelial cell FHC cells were treated with LPS (200 ng/ml) in vitro, and the mRNA and protein expression of EGFR was detected and analyzed at different time points. (B–C) FHC cells were transfected with miR-7 inhibitors/NC (200 nM) following LPS treatment, 48 h later, Real-time PCR analysis for miR-7, EGFR, and inflammatory cytokines IL-6, TNF-α, TGF-β, IL-10, CSF4, CCL4, CXCL3, and CX3CL1. The expression of phos-NF-kB, phos-EGFR, and Ki-67 were observed by multiplexed fluorescent immunohistochemical staining (D). (E) FHC cells were transfected with miR-7 inhibitors / miR-7 inhibitors plus Erlotinib (5 nM) following LPS treatment, the relative expression of inflammatory cytokines IL-6, TNF-α, TGF-β, IL-10, CSF4, CCL4, CXCL3, and CX3CL1 was detected and analyzed by Real-time PCR; and the expression of phos-NF-kB, phos-EGFR, and Ki-67 was observed by multiplexed fluorescent immunohistochemical staining (F); (G) Immunoblot analysis of p-EGFR, EGFR, PI3K, p-PI3K, NF-kB, p-NF-kB, AKT, p-AKT, ERK, p-ERK, Ras and PDK1 in the cells of each group; (H) The cell migration was assessed by transwell assay. The values are the means ± SD (n = 3). *P < 0.05, **P < 0.01.