Fig. 8.

Intestinal epithelial cell-specific miR-7 silencing alleviates the pathology of IBD. (A) Schematic representation of the establishment of WT IBD mice challenged with the p-v-miR-7sp vector. p-v-miR-7sp vector was injected into WT mice (8–10 W, n = 6/per group) via tail vein at an interval of 5 mg/kg, every 2 days, for 3 times, and establish IBD model, then the body weight loss (B) and the survival curve (C) were analyzed; Representative gross images of colon were observed (D); Histopathology was performed by H&E staining and mucin production was measured by Alcian blue staining (×100, ×200) (E); IF analysis of CD45⁺ cell and Ki-67 expression in colorectal tissue (×200, ×400) (F–G); FCM analysis of CD45⁺ cells and CD4⁺ T cells, CD8⁺ T cells, CD19⁺ B cells, Gr-1⁺ cells, CD11b⁺ cells and NK1.1⁺ T cells in CD45⁺ cells (H–I); Real-time PCR analysis of cytokines IL-6, TNF-α, TGF-β, IL-10, CSF4, CCL4, CXCL3 and CX3CL1 in colon (J); the relative expression of miR-7 was detected by Real-time PCR in colon tissue (K); IF analysis for p-NF-κB, EGFR and EPCAM in colon (×0.5, ×630) (L). The values are the means ± SD (n = 6). *P < 0.05, **P < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)