FIG. 1.

(A) The upper sequence shows the amino acid sequence of TIF2. Differences in hTIF2 are shown below this sequence. IAD wt is marked by a grey box. The three NR boxes located within the IAD are marked by black boxes. The three NR boxes, beginning with the N-terminal one, are NR box 1, NR box 2, and NR box 3, respectively. (B) Different variants of the IAD wt were constructed by replacement of two Leu residues by Ala residues within the LXXLL motif as indicated. By mutation of only one box within the otherwise intact IAD, we generated variants named Mut 1, 2, and 3. Mutations of two boxes led to variants harboring only one functional box, named boxes 1, 2, and 3. The variant carrying mutations of all three NR boxes was named IAD Mut 123.