FIG. 6.

The three NR boxes within the IAD of TIF2 bind preferentially to different receptors. Mutated variants of the IAD of TIF2 were fused to the GAD and tested in the presence of the corresponding hormones in a yeast two-hybrid assay for interaction with the LBDs of PPARα (A), TRα (B), GR (C), and RXRβ (D). (Left panels) Effects of point mutations of single modules (Mut 1, Mut 2, and Mut 3) within the IAD compared to IAD wt, defined as 100%. (Right panels) Effects of variants carrying only single modules (box 1, box 2, and box 3) within the IAD compared to IAD wt, defined as 100%.