Table 2. Pharmacokinetic parameters of Ex-POEGMA_opt.

Pharmacokinetic parameters were calculated from the data given in Figure 4. Data were fitted to a one-phase exponential decay curve and analyzed via non-compartmental pharmacokinetic analysis using GraphPad Prism 8 software and MRT values were calculated within WinNonlin software. Absorption phase pharmacokinetics of Ex-PEG_Rh in immunized mice were not calculated (N/C) due to its low goodness-of-fit values. Data showed the mean ± SEM.

	Treatment	t1/2 absorption (h−1)	t1/2 elimination (h−1)	MRT (h)	tmax (h)*	Cmax (nM)*	AUC (h × nM)
Naïve Mice	Exendin	0.65 ± 0.04	2.12 ± 0.3	4.2 ± 0.1	1.6 ± 0.1	31.7 ± 7.5	118 ± 9
	Ex-PEGRh	6.59 ± 0.27	23.14 ± 3.2	29.8 ± 2.4	16.6 ± 1.1	34.6 ± 1.2	1585 ± 56
	Ex-PEGMw	6.6 ± 0.9	34.91 ± 4.5	38.1 ± 2.2	19.5 ± 2.5	35.3 ± 2.2	2206 ± 42
	Ex-POEGMAopt	9.1 ± 0.9	97.3 ± 3.2	59.8 ± 6.6	32.1 ± 2.9	35.8 ± 1.3	3664 ± 83
Immunized Mice	Exendin	0.77 ± 0.07	1.88 ± 0.2	4.9 ± 0.4	1.72 ± 0.1	36.9 ± 3.4	159 ± 9
	Ex-PEGRh	N/C	11.9 ± 2.7	24.0 ± 2.5	N/C	N/C	424 ± 17
	Ex-POEGMAopt	12.1 ± 1.0	105.7 ± 6	70.8 ± 0.2	42.6 ± 3.2	30.3 ± 2.0	3664 ± 83