FIG. 1.

Antigen for MAb production. The in vitro RNA editing activity was enriched by subcellular fractionation of mitochondria followed by (NH₄)₂SO₄ precipitation and glycerol gradient fractionation to prepare immunogen used for the production of MAbs (see Materials and Methods). (A) In vitro editing assay (U deletion) of fractions from sequential steps of (NH₄)₂SO₄ precipitation of crude mitochondrial lysate, showing editing activity in the 45% pellet. The product formed by the deletion of three U’s from editing site 1 of the input RNA (A6short/Tag.1) is indicated. (B) In vitro RNA editing assay (U deletion) of the 20S fraction from glycerol gradient sedimentation of the 45% (NH₄)₂SO₄ precipitate showing the editing activity.