FIG. 5.

Immunoprecipitation of in vitro editing activity. Material that immunoprecipitates with MAbs against gBP21 was tested for its ability to support in vitro RNA editing that deletes (A) or inserts (B) U’s. The right gel in panel B shows a longer separation of the RNA molecules that increases the resolution of the edited product from the input RNA. Input RNA, 3′ cleavage product, chimeras, and edited product are diagramed to the right of the corresponding RNA molecule. Editing products were identified by their comigration with known editing products. Editing activity is immunoprecipitated with magnetic beads coupled with MAbs specific for gBP21 but not with immunomagnetic beads alone (No MAb) or with immunomagnetic beads coupled to a control antibody (ODB2).