FIG. 6.

Immunoprecipitation of TUTase and [α-³²P]ATP self-adenylylatable proteins (RNA ligase). (A) TUTase activity, as monitored by the addition of [α-³²P]UTP to yeast tRNA (counts per minute are shown), immunoprecipitates with the anti-gBP21 MAbs but not with immunomagnetic beads alone (No MAb) or immunomagnetic beads coupled with control MAb ODB2. (B) Anti-gBP21 MAbs immunoprecipitate the [α-³²P]ATP self-adenylylatable proteins (RNA ligase) while immunomagnetic beads and the control antibody (ODB2) do not.