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. 2024 Mar 7;17:37. doi: 10.1186/s13068-024-02473-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — 3D surface plots for the response lignin reduction (%) due to EnZolv pretreatment in blowroom droppings. A Effect of moisture content vs. enzyme load; B effect of moisture content vs. incubation time; C effect of moisture content versus incubation temperature; D effect of moisture content vs. shaking speed; E effect of enzyme load vs. incubation time; F effect of enzyme load vs. incubation temperature; G effect of incubation time vs. incubation temperature; H effect of incubation temperature vs. shaking speed. The interactive effect is represented with color ranging from blue to red (blue, green, red); blue is least significant, green is moderately significant, and red is highly significant