Fig. 6.

BcRAE and BcFAT induce cell death depending on SGNH hydrolase activity. A SDS-PAGE analysis of native and mutant proteins. The positions of the target proteins were marked with black arrows. B Treatment of tobacco leaves with 50 μM CK, native BcRAE and BcFAT, and mutant proteins. BcRAE-S28A: the Ser28 residue of BcRAE was mutated to Ala. BcFAT-S87A: the Ser87 residue of BcFAT was mutated to Ala. Photos were taken 3 d after treatment. Black dashed circles indicate infiltrated sites. C Effect of high-temperature denaturation on the cell death-inducing activities of BcFAT and BcRAE. Prokaryotically expressed proteins were incubated at 95 ℃ for 15 min and infiltrated into tobacco leaves, and proteins treated at 25 ℃ were infiltrated as the control. Photos were taken 3 d after infiltration. Black dashed circles indicate infiltrated sites