Figure 9.

Three-dimensional reconstruction of tumor-associated vessels indicates a normalized vessel structure in mice harboring TGF-β-KO GBMs as well as in mice with PAR tumors that received Lenti-SLUG-KO. 3D reconstruction after image improvement using Imaris. ECs (CD31⁺, green) were shown within the tumor region in RGS5-GFP reporter mice bearing either parental GL261 derived GBMs (red) without further treatment (PAR, upper panel), of an animal bearing a TGF-β-KO GBM (TGF-β-KO, middle panel), and of a mouse bearing a PAR tumor and that have been intrastriatally injected with Lenti-SLUG-KO (SLUG-KO, lower panels). Black/white pictures (right side) showing vascular alterations (triangles exemplary show glomeroid-like vessel structures and arrows normal branches). All images are taken at 20x magnification (n=1 mouse per group; scale bars = 50 µm).