Key resource table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Critical commercial assays | ||
| Deposited data | ||
| Experimental images and flow cytometry data | This paper | https://github.com/Weiss-Lab/synthetic-symmetry-breaking, version of record deposited at Zenodo at: https://doi.org/10.5281/zenodo.8067436 |
| Experimental models: Cell lines | ||
| cNW2.2: reporter cell line described in SI Fig 1A to generate data shown in Fig 1D & SI Fig 2 | This paper | N/A |
| mCE40m4: colors-only symmetry breaking cell line described in SI Fig 1C to generate data shown in Fig 2B–C & SI Fig 3–17 | This paper | N/A |
| cNW1m4: colors + cadherins symmetry breaking & shape formation cell line described in SI Fig 1D to generate data shown in Fig 3B–C, Fig 4D, and SI Fig 18–21 | This paper | N/A |
| cNW1m3: colors + cadherins symmetry breaking & shape formation cell line generating data shown in SI Fig 23 (top row) | This paper | N/A |
| cNW1m6: colors + cadherins symmetry breaking & shape formation cell line generating data shown in SI Fig 23 (bottom row) | This paper | N/A |
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| pJT87 (Wβ expression) | This paper | Addgene ID 204489 |
| pJT88 (φC31 expression) | This paper | Addgene ID 204490 |
| pJTT002 (colors-only switch responsive to recombinases used in cell line shown in Figure 2) | This paper | Addgene ID 204493 |
| aCE89 (ABA-ON φC31, Dox-ON Wβ) | This paper | Addgene ID 204494 |
| aCE90 (ABA-ON φC31-PEST, Dox-ON Wβ-PEST) | This paper | Addgene ID 204495 |
| aCE106 (PhlF-NES-ABI, NLS-VPR-Pyl1, & rtTA3 expression) | This paper | Addgene ID 204496 |
| aCE120 (colors-only switch responsive to recombinases used in cell line shown in Figure 1) | This paper | Addgene ID 204491 |
| aCE121 (colors& cadherins switch responsive to recombinases used in cell line shown in Figures 3–4) | This paper | Addgene ID 204492 |
| Software and algorithms | ||
| Analysis code for experimental images & flow cytometry | This paper | https://github.com/Weiss-Lab/synthetic-symmetry-breaking, version of record deposited at Zenodo at: https://doi.org/10.5281/zenodo.8067436 |
| Python ABM library | This paper | https://github.com/kemplab/pythonabm, version of record deposited at Zenodo at: https://doi.org/10.5281/zenodo.8007463 |
| 3D cadherin ABM to simulate symmetry breaking & multicellular structure formation | This paper | https://github.com/kemplab/cho-adhesion-model, version of record deposited at Zenodo at: https://doi.org/10.5281/zenodo.8007620 |
| ImageJ | Schneider et al.72 | https://imagej.net/ij/index.html |
| MATLAB | https://www.mathworks.com/products/matlab.html | |
| Other | ||