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. 1978 Apr;61(4):639–643. doi: 10.1104/pp.61.4.639

Phase Behavior of Chloroplast and Microsomal Membranes during Leaf Senescence 1

Bryan D McKersie 1, John E Thompson 1
PMCID: PMC1091934  PMID: 16660353

Abstract

Wide angle x-ray diffraction of chloroplast and microsomal membranes from primary leaves of Phaseolus vulgaris has revealed that for both types of membrane, portions of the lipid become crystalline as the tissue senesces. For young leaves the transition temperature is about 23 C for microsomes and below −30 C for chloroplast membranes, indicating that at physiological temperature the lipid is entirely liquid-crystalline. Between 2 and 3 weeks after planting the transition temperature rises to 38 C for microsomes, but for chloroplasts this increase to a point above physiological temperature does not occur until between 3 and 4 weeks. Thereafter the transition temperature continues to rise for both types of membrane with advancing senescence, although the rate of increase is greater for chloroplasts than for microsomes. The appearance at physiological temperature of gel phase lipid in the microsomes coincides temporally with the initiation of a decline in total protein in the tissue, and the incidence of crystallinity in chloroplasts coincides with loss of chlorophyll. This change in phase behavior cannot be attributed to an alteration in fatty acid composition, but for both membrane systems it correlates with an increase of about 4-fold in the sterol to phospholipid ratio.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandt R. D., Benveniste P. Isolation and identification of sterols from subcellular fractions of bean leaves (Phaseolus vulgaris). Biochim Biophys Acta. 1972 Sep 1;282(1):85–92. doi: 10.1016/0005-2736(72)90313-6. [DOI] [PubMed] [Google Scholar]
  2. Demel R. A., Jansen J. W., van Dijck P. W., van Deenen L. L. The preferential interaction of cholesterol with different classes of phospholipids. Biochim Biophys Acta. 1977 Feb 14;465(1):1–10. doi: 10.1016/0005-2736(77)90350-9. [DOI] [PubMed] [Google Scholar]
  3. Esfahani M., Limbrick A. R., Knutton S., Oka T., Wakil S. J. The molecular organization of lipids in the membrane of Escherichia coli: phase transitions. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3180–3184. doi: 10.1073/pnas.68.12.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grunwald C. Sterol distribution in intracellular organelles isolated from tobacco leaves. Plant Physiol. 1970 Jun;45(6):663–666. doi: 10.1104/pp.45.6.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Ladbrooke B. D., Williams R. M., Chapman D. Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction. Biochim Biophys Acta. 1968 Apr 29;150(3):333–340. doi: 10.1016/0005-2736(68)90132-6. [DOI] [PubMed] [Google Scholar]
  7. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  8. McKersie B. D., Thompson J. E. Lipid crystallization in senescent membranes from cotyledons. Plant Physiol. 1977 May;59(5):803–807. doi: 10.1104/pp.59.5.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Papahadjopoulos D., Jacobson K., Nir S., Isac T. Phase transitions in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol. Biochim Biophys Acta. 1973 Jul 6;311(3):330–348. doi: 10.1016/0005-2736(73)90314-3. [DOI] [PubMed] [Google Scholar]
  10. Papahadjopoulos D., Moscarello M., Eylar E. H., Isac T. Effects of proteins on thermotropic phase transitions of phospholipid membranes. Biochim Biophys Acta. 1975 Sep 2;401(3):317–335. doi: 10.1016/0005-2736(75)90233-3. [DOI] [PubMed] [Google Scholar]
  11. Roughan P. G., Boardman N. K. Lipid Composition of Pea and Bean Leaves during Chloroplast Development. Plant Physiol. 1972 Jul;50(1):31–34. doi: 10.1104/pp.50.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shechter E., Letellier L., Gulik-Krzywicki G. Relations between structure and function in cytoplasmic membrane vesicles isolated from an Escherichia coli fatty-acid auxotroph. High-angle x-ray diffraction, freeze-etch electron microscopy and transport studies. Eur J Biochem. 1974 Nov 1;49(1):61–76. doi: 10.1111/j.1432-1033.1974.tb03811.x. [DOI] [PubMed] [Google Scholar]
  13. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  14. Van Dijck P. W., Ververgaert P. H., Verkleij A. J., Van Deenen L. L., De Gier J. Influence of Ca2+ and Mg2+ on the thermotropic behaviour and permeability properties of liposomes prepared from dimyristoyl phosphatidylglycerol and mixtures of dimyristoyl phosphatidylglycerol and dimyristoyl phosphatidylcholine. Biochim Biophys Acta. 1975 Nov 3;406(4):465–478. doi: 10.1016/0005-2736(75)90025-5. [DOI] [PubMed] [Google Scholar]

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