FIG. 1.
Deletion analysis of BAX by yeast two-hybrid system. Various BAX NH2- or COOH-terminal truncations in DBD vector pBTM were tested against BAX, BCL-2, BCL-XL, and MCL-1 in the AD vector pACTII. Pairs of DBD and AD vectors were transformed into yeast strain L40 and plated on medium without Trp or Leu. Grown-up colonies were transferred onto NitroPure filters and assayed for β-Gal activity. −, no interaction; ±, weak interaction; + and ++, strong interaction; ND, no data.