FIG. 5.

BAX-induced apoptosis in Jurkat cells. Jurkat clones with inducible expression of wt BAX or HABAXmIII-1 were treated with 1 μg of doxycycline per ml and assayed for PI exclusion (A), mitochondrial-membrane potential (B), and ROS production (C) at the indicated time points. Data shown are means ± standard deviations (error bars) from three independent experiments. (D) Caspase activity was analyzed with fluorescent peptide substrate DEVD-AFC. (E) Release of cytochrome c (cyto c) was assessed by Western blot hybridization of subcellular fractions with an anti-cytochrome c MAb. HE, hydroethidine; Eth, ethidium.