TABLE 1.
Yeast two-hybrid analysis of the protein interactions of BAX BH3 mutantsa
| BAX wt or mutant construct in DBD vector pBTM | Protein interaction with indicated construct in AD vector pACTIIb
|
||
|---|---|---|---|
| BAX | BCL-2 | BCL-XL | |
| BAXwt | + | + | + |
| mIII-1 | − | − | − |
| mIII-2 | − | − | − |
| mIII-3 | + | + | + |
| mIII-4 | ± | − | − |
| mIII-5 | + | − | − |
| mIII-6 | + | + | + |
| mIII-7 | + | ND | + |
| mIII-8 | + | + | + |
| mIII-9 | + | + | ± |
| mIII-10 | + | + | ± |
| mIII-11 | + | + | − |
| mIII-12 | + | + | − |
| mIII-13 | + | + | + |
| mIII-14 | + | + | + |
| mIII-15 | + | + | + |
a
All constructs lack COOH-terminal signal anchor (ΔC). Results reflect β-Gal assays from at least two independent experiments.
b
+, strong interaction; −, no interaction; ±, weak interaction; ND, no data.