FIGURE 1.

Accumulation of PC3MLuc EVs over 27 days in adult male CD1 NuNu mice. Accumulation of Dil labelled PC3MLuc EVs was determined via IVIS imaging at 1, 7-, 13-, 20- and 27-day timepoints in adult CD1 NuNu male mice exposed to EVs twice per week, compared to a vehicle only control. (A) brain, (B) heart, (C) lung, (D) liver, (E) spleen, (F) pancreas, (G) kidney, (H) lymph nodes, (I) bladder, (J) urine, and (K) limb bones. (L, M) IVIS imaging of organs and bones at days 13 (L) and 27 (M) in CD1 mice exposed to PC3MLuc EVs. Accumulation of EV signal can be seen in the joint area of the limbs, changing from low to high signal detection in the limbs of 27-day animals. Statistical significance was determined by 2-way ANOVA with Dunnett’s multiple comparison test, with single pooled variance. *<0.05, **<0.01, ***<0.001, ****<0.0001 (n = 2).