Fig 1. Magnetic Resonance Imaging (MRI)-histological correspondence and microglial cell density in the tumor, its surroundings, the peritumoral brain zone (PBZ) and control tissue peritumoral brain zone (PBZ) localization.

(A) Gadolinium-enhanced MRI sample of a coronal brain gadolinium-enhance section containing the tumor. The PBZ is delimited by a double-headed arrow. (B) Mosaic reconstruction of the Iba-1-immunostained/Nissl counterstained slice matching the MRI sample. Scale bar represents 2 mm. Both images exhibit the four regions of interest (ROI; T = tumor, I = interface, PBZ, CL = contralateral hemisphere). (C) Iba-1-immunostained/Nissl counterstained tumor and its surroundings (10X objective). (D) Tumor volume for each animal compared to its contralateral hemisphere. (E) Quantification of the microglia density in an area of 25.96 x 10³ μm² for each ROI. *P<0.05, **P<0.01, #P<0.001. Representative photomicrographs (40X objective; Scale bar represents 50 μm) of Iba-1-immunostained/Nissl counterstained (F) tumor, (G) interface, (H) PBZ and (I) CL areas are also shown.