Skip to main content
. 1998 Oct;18(10):6102–6109. doi: 10.1128/mcb.18.10.6102

FIG. 4.

FIG. 4

Coimmunoprecipitation of Dpb11 and Sld2. Dpb11 W and Dpb11 11-t, plasmids that express Gal4-HA-Dpb11 and Gal4–HA–Dpb11-t truncated proteins, respectively. Sld2 W and Sld2 2-6, plasmids that express LexA-Sld2 and LexA–Sld2-6 mutant proteins, respectively. Proteins extracted from yeast strain CB001 expressing each pair of the plasmids were precipitated with an anti-HA monoclonal antibody (12CA5). The protein extracts and the precipitates were analyzed for the presence of LexA-Sld2 or LexA–Sld2-6 probed with anti-LexA serum (A) and for the presence of Gal4-HA-Dpb11 or Gal4–HA–Dpb11-t probed with anti-HA monoclonal antibody (12CA5) (B). In lanes 1, 3, 5, and 7, the soluble protein extracts before immunoprecipitation were applied. In lanes 2, 4, 6, and 8, the immunoprecipitates were applied.