FIG. 5.

DNA replication in dpb11-1 and sld2-6 cells. (A) YHA410 (DPB11) and YHA411 (dpb11-1) cells were arrested with α-factor at 25°C and then released at 37°C as described in Materials and Methods. Aliquots were withdrawn at the indicated times to measure total DNA by the diphenylamine method (50). (B) YYK6 (SLD2) and YYK7 (sld2-6) cells were synchronized, and the total DNA at 37°C was measured as for panel A. The delay of DNA synthesis in YYK6 and YYK7 is probably caused by delayed release from α-factor (see Fig. 6).