TABLE 3.
Nuclear division after release from an HU block
| Strain | % of cells having two nucleia at:
|
||
|---|---|---|---|
| 0 min | 80 min | 240 min | |
| YYK6 | 2 | 52 | NDb |
| YYK7 (sld2-6) | 8 | 6 | 5 |
| YHA410 | 0 | 58 | ND |
| YHA411 (dpb11-1) | 5 | 8 | 52c |
a
Cells were synchronized with α-factor, released to YPD medium containing 0.2 M HU at 25°C for 90 min, and then transferred to 37°C. After 30 min of incubation, cells were washed and released from the HU block at 37°C. At the indicated time after release from HU, nuclei were observed.
b
ND, not determined.
c
Most of these cells have two nuclei with the abnormal morphology described previously (6).