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. 2024 Feb 26;18(2):e0011960. doi: 10.1371/journal.pntd.0011960

Fig 3. Surface protein expression of CD127 and CD132 on CD4+ T cells from endemic controls (ECs) and visceral leishmaniasis (VL) patients.

Fig 3

A. Gating strategy for CD4+ T cell flow cytometry analysis. B. Percentage (top) and mean fluorescent intensity (MFI) (bottom) of CD127 on CD4+ T cells. C. Percentage (top) and MFI (bottom) of CD132 on CD4+ T cells. D. Percentage (top) and MFI (bottom) of CD38 on CD4+ T cells. Statistical significance between ECs (n = 11) and VL patients (n = 12) was determined by Mann-Whitney U-test in Fig 3B-D, and 3F are indicated as *p<0.05; **p<0.01. E. Boolean-Gating (FlowJo), was used to define complex cell sub-population, and Simplified presentation of incredibly complex evaluations (SPICE) polyfunctionality analysis. SPICE was used to establish overlap in expression of CD38, CD127 and CD132 on CD4+ T cells. Pie charts represent the entire CD4+ T cell population expressing either CD38, CD127, CD132 or none of them. Data was generated from EC (n = 7) and VL patients (n = 7). F. Percentage and MFI of CD127, and CD132, expression on CD38+/- CD4 T+ cells of VL (n = 7) and EC (n = 7).