Table 1. Kinetic parameters of murine Cpeb3 ribozyme constructs^†.

Construct*	A	k1	B	k2	C
–10/72	0.72 ± 0.09	0.39 ± 0.09			0.082 ± 0.026
–49/72/165	0.88 ± 0.02	0.42 ± 0.04	0.013 ± 0.015	0.11 ± 0.03	0.04 ± 0.02
–233/72/165	0.78 ± 0.04	0.31 ± 0.04	0.035 ± 0.006	0.17 ± 0.02	0.029 ± 0.005

^*Construct size is defined as (length of sequence upstream of the ribozyme cleavage site)/[Cpeb3 ribozyme (72 nts)]/(downstream sequence).

^†Co-transcriptional self-scission was modeled by a bi-exponential decay model with a residual. A and B represent fractions of the population cleaving with fast (k₁) and slow (k₂) rate constants, cleave. Errors represent SEM of at least three experiments. For the smallest ribozyme construct (-10/72), a monoexponential decay function was sufficient to model the data.