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Basic PCR system.
| Basic system | N | A | E | F |
|---|---|---|---|---|
| Composition | Volume (μL) | Volume (μL) | Volume (μL) | Volume (μL) |
| DNA polymerase | 0.5 | 25 | 0.75 | 2 |
| RT enzyme mix | 2.5 | 25 | 0.4 | 2 |
| RNase inhibitor | 2.5 | 25 | 0.5 | 10 |
| Mg2+(25 mM) | 3 | 25 | 16 | 10 |
| Reaction buffer | 9 | 25 | 16 | 10 |
| Upstream primer (10 μM) | 1 | 1 | 1 | 1 |
| Downstream primer (10 μM) | 1 | 1 | 1 | 1 |
| Probe (10 μM) | 0.5 | 0.5 | 0.5 | 0.5 |
| Sample | 5 | 5 | 5 | 5 |
| Enzyme-free sterile water | Fill in up to 50 μL | Fill in up to 50 μL | Fill in up to 50 μL | Fill in up to 50 μL |
