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Configuration of different amplification procedures.
| Reaction phase | Temperature | Duration | |||
|---|---|---|---|---|---|
| Amplification procedure 1 (conventional amplification procedure) | Amplification procedure 2 (rapid amplification procedure) | ||||
| Degradation U-templates | 25°C | 10 min | 2 min | ||
|
| |||||
| Reverse transcription | 50°C | 15 min | 3 min | ||
|
| |||||
| Pre-denaturation | 95°C | 5 min | 30 s | ||
|
| |||||
| Denaturation | 95°C | 15 s | 40 cycles | 3 s | 40 cycles |
| Annealing and extension | 60°C | 30 s | 5 s | ||
|
| |||||
| Total duration | ∼1 h 30 min | ∼30 min | |||
