Table 4.

Ct values under different concentrations of primers and probes.

		Primer concentration (μM)
		0.05	0.1	0.15	0.2	0.25	0.3	0.35	0.4
SARS-CoV-2 NP probe concentration (μM)	0.05	33.24	32.97	33.34	33.22	33.08	33.20	33.04	33.18
	0.10	32.29	32.26	32.33	32.28	32.39	32.20	32.20	32.22
	0.15	32.01	31.86	32.15	31.98	32.17	31.94	32.06	31.97
	0.20	32.50	32.09	32.00	32.02	31.98	32.20	31.75	32.11
SARS-CoV-2 ORF probe concentration (μM)	0.05	34.14	34.04	34.92	36.63	35.54	39.21	N/A	N/A
	0.10	34.20	33.21	33.84	34.98	35.39	37.09	39.82	N/A
	0.15	33.19	32.24	33.86	33.73	34.09	36.70	39.31	N/A
	0.20	33.72	32.62	35.90	35.14	34.31	35.76	39.69	N/A
FluA M probe concentration (μM)	0.05	31.01	30.69	30.62	30.71	30.61	30.76	30.81	31.07
	0.10	30.55	30.27	30.02	30.18	29.98	29.73	29.93	29.98
	0.15	30.25	30.02	29.67	29.71	29.45	29.57	29.60	29.75
	0.20	30.54	29.91	29.62	29.69	29.61	29.11	29.40	29.63
FluB NS probe concentration (μM)	0.05	33.68	33.29	32.66	33.20	32.33	32.88	32.47	32.60
	0.10	33.07	32.77	32.54	32.72	32.67	32.21	32.25	32.76
	0.15	32.97	33.38	32.46	32.53	32.01	32.53	32.33	32.60
	0.20	34.03	32.55	32.63	32.68	32.62	32.98	33.02	32.87