Fig. 5.

Complementation of the Arabidopsis ahk2/3 loss-of-function mutant via PgHK3 overexpression. (A) Phenotype of the CK-insensitive dwarf phenotype of the ahk2/3 double mutant and overexpression of PgHK3 and PgHK3-H442Q genes in the ahk2/3 background. Scale bar = 1cm. (B) Relative expression level of PgHK3, PgHK3-H442Q in 7-day-old- transgenic plants. Different lowercase letters indicate statistically significant differences P < 0.05; one-way ANOVA, followed by Tukey's multiple range test. Cross-section images of (C) stem of the transgenic plants. (D)The vascular bundle phenotype of PgHKH442Q overexoression line in Col-0 background. Scale bar = 100 μm.