TABLE 2.
Calculated p-values resulting from one-way ANOVAS to validate changes in fitness relative to the WT in broth (Table 2A - DMB vs DMB and Table 2D - DMB vs. LB and Table 2E - LB vs LB). One-way ANOVAs were also used to determine if the changes in fitness were the result of the specific genotype (Table 2B - DMB and Table 2F - LB) by comparing each mutant to the WT in the same respective silver concentration, or due to the presence of silver nitrate (Table 2C - DMB and Table 2G - LB) by comparing each population to itself in absence of silver nitrate.
| A | vs. WT in DMB [0] | B | vs. [WT] in DMB | C | vs. mutant @ [0] in DMB | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DMB | DMB | DMB | |||||||||||||||
| [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H | [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H | [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H |
| 0 | >0.9999 | 0.9899 | 0.9312 | 0.828 | 0.1167 | 0 | >0.9999 | 0.9813 | 0.9092 | 1 | 0.1314 | 0 | >0.9999 | >0.9999 | >0.9999 | >0.9999 | >0.9999 |
| 5 | 0.0033 | 0.0137 | 0.0331 | 0.9511 | <0.0001 | 5 | >0.9999 | 0.9823 | 0.8932 | 0.0006 | <0.0001 | 5 | 0.0298 | 0.0001 | 0.0843 | 0.6449 | 0.0011 |
| 50 | <0.0001 | 0.9449 | 0.0029 | 0.6868 | 0.1006 | 50 | >0.9999 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 50 | <0.0001 | 0.9964 | 0.0025 | 0.9986 | 0.9679 |
| 60 | <0.0001 | <0.0001 | >0.9999 | <0.0001 | <0.0001 | 60 | >0.9999 | <0.0001 | <0.0001 | 0.0668 | 0.7069 | 60 | <0.0001 | <0.0001 | 0.998 | <0.0001 | <0.0001 |
| 70 | 0.9998 | >0.9999 | 0.9399 | <0.0001 | <0.0001 | 70 | >0.9999 | 0.998 | 0.8392 | <0.0001 | <0.0001 | 70 | >0.9999 | 0.9777 | 0.7044 | <0.0001 | <0.0001 |
| 80 | <0.0001 | 0.9781 | 0.081 | <0.0001 | <0.0001 | 80 | >0.9999 | <0.0001 | <0.0001 | 0.0006 | 0.1805 | 80 | <0.0001 | >0.9999 | 0.5936 | <0.0001 | <0.0001 |
| 90 | <0.0001 | 0.0038 | <0.0001 | <0.0001 | <0.0001 | 90 | >0.9999 | <0.0001 | 0.0129 | 0.8303 | 0.1273 | 90 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| 100 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 100 | >0.9999 | 0.0496 | 0.7324 | 0.0318 | <0.0001 | 100 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| D | vs. WT in DMB [0] | E | vs. WT in LB [0] | F | vs. [WT] in LB | G | vs. mutant @ [0] in LB | ||||||||||||||||
| LBvsDMB | LBvsLB | LBvsLB | LBvsLB | ||||||||||||||||||||
| [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H | [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H | [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H | [silver nitrate] ng/mL | WT | R15L | T14P | T17P | N279H |
| 0 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0 | >0.9999 | 0.0007 | 0.0041 | 0.0267 | <0.0001 | 0 | >0.9999 | 0.0007 | 0.0041 | 0.0267 | <0.0001 | 0 | >0.9999 | >0.9999 | >0.9999 | >0.9999 | >0.9999 |
| 100 | <0.0001 | <0.0001 | <0.0001 | 0.001 | <0.0001 | 100 | 0.1012 | 0.686 | 0.0044 | 0.0001 | 0.0006 | 100 | >0.9999 | 0.0011 | 0.5699 | <0.0001 | <0.0001 | 100 | 0.1538 | 0.0007 | <0.0001 | <0.0001 | >0.9999 |
| 250 | <0.0001 | <0.0001 | <0.0001 | 0.0004 | <0.0001 | 250 | 0.1265 | 0.0043 | 0.0033 | 0.0001 | 0.4744 | 250 | >0.9999 | <0.0001 | <0.0001 | <0.0001 | 0.0001 | 250 | 0.3331 | 0.9957 | >0.9999 | <0.0001 | <0.0001 |
| 500 | <0.0001 | <0.0001 | <0.0001 | 0.0259 | <0.0001 | 500 | 0.1595 | 0.9717 | 0.0907 | 0.0001 | 0.3364 | 500 | >0.9999 | 0.3363 | <0.0001 | 0.0007 | 0.9817 | 500 | 0.1732 | <0.0001 | <0.0001 | <0.0001 | 0.0026 |
| 750 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 750 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0291 | 750 | >0.9999 | 0.0001 | 0.0002 | 0.0301 | <0.0001 | 750 | <0.0001 | 0.9822 | 0.6858 | <0.0001 | 0.0053 |
| 1,000 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 1,000 | 0.0143 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 1,000 | >0.9999 | 0.0015 | 0.371 | <0.0001 | 0.0283 | 1,000 | 0.191 | 0.511 | >0.9999 | <0.0001 | 0.9977 |
| 2,500 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 2,500 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 2,500 | >0.9999 | 0.9746 | 0.9997 | 0.0132 | 0.427 | 2,500 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| 5,000 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 5,000 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 5,000 | >0.9999 | 0.0031 | 0.0005 | <0.0001 | <0.0001 | 5,000 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| 10,000 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 10,000 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 10,000 | >0.9999 | >0.9999 | <0.0001 | <0.0001 | <0.0001 | 10,000 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
The population used for comparison in the one-ways ANOVAs for the mutants and conditions.