Figure 2.

Cell type-specific uptake of various DNA molecules by FACS analysis. (A, B) Quantification of percent positive cells treated with six different Cy5 labeled DNA sequences (Ctr, MM-ZC, MM1, MM3, MM4, PC1) after 4hr of the treatments. Percent positive cells are on Y-axis. DNA treatments are on X-axis. * Indicates p<0.05 and ** indicates p<0.01 relative to control (cells only). Trypsin-treated and untreated cells are represented as yellow and blue bars, respectively. A = MM1R; B = SKO-007. Error bars represent the standard error of means (SEM) from 3 replicates. Sample size for each dose point is at least 10K. Unpaired T-test is used to determine the significant difference. (C-F) Representative confocal images (selected from ten random different field of view) of JK6L (C, D) and U266B1 (E, F) cells showing the different uptake amounts of Rhodamine-labeled Ctr (top panel) MM-ZC (bottom panel). The red and green fluorescence signal represents the DNA and phalloidin (membrane) staining respectively; the blue indicates the DAPI stained Nuclei. Images were taken after 4 hr of treatment with 100 ng/ml of the MM-ZC. Scale bar 10 μm. Images are captured by using Zeiss 700 confocal microscopy.