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N-homocysteinylation degree of nisin under various HTL concentrations and pHs at 45 °C, 150 rpm shaker rate, and 6 mg/mL nisin concentration
| HTL concentration (mM) | pH | N-homocysteinylation degree (%)* |
|---|---|---|
| 100 | 3.0 | 6.07±0.002 |
| 4.0 | 6.15±0.001 | |
| 4.5 | 5.53±0.002 | |
| 5.0 | 5.73±0.002 | |
| 150 | 3.0 | 6.30±0.020 |
| 4.0 | 6.22±0.021 | |
| 4.5 | 5.86±0.008 | |
| 5.0 | 5.74±0.050 | |
| 200 | 3.0 | 5.96±0.035 |
| 4.0 | 6.09±0.015 | |
| 4.5 | 5.85±0.030 | |
| 5.0 | 5.73±0.036 |
*Mean ± standard deviations, HTL homocysteine thiolactone
