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Steps and conditions with time duration for the thermocycler of polymerase chain reaction (PCR)
| Steps | Temperature (°C) | Time (min) | Number of cycle |
|---|---|---|---|
| Pre-denaturation | 94 | 5 | 1 |
| Denaturation | 94 | 1 | 30 |
| Primer annealing | 57 | 0.5 | 30 |
| Extension | 72 | 1 | 30 |
| Final extension | 72 | 10 | 1 |
