Table 2.

Temperature profile used in PCR for the amplification of fliC gene with primer pair FliC-FP and RP

Step	Temperature (°C)	Time (minutes)	No. of cycles
Initial denaturation	95	3	1
Denaturation	95	0.5	35
Annealing	60	0.5
Extension	72	0.7
Final extension	72	3	1