FIG. 6.

(A) Schematic representation of the Gal4 fusion proteins containing the HOXB1 N-terminal domain (positions 1 to 164) or its deletion mutants (positions 38 to 164 and 90 to 164), and of the pTUAS luciferase reporter plasmid. Solid boxes indicate the Gal4 1 147 DBD. (B) Transcriptional activity of the HOXB1-Gal4 chimeras in COS7 cells transfected with 2 μg of reporter plasmid (UAS) and cotransfected with 2 to 6 μg of B1(1–164)-Gal4, B1(38–164)-Gal4, B1(90–164)-Gal4, and Gal4-DBD. Luciferase activity is expressed as fold activation over the basal activity of the promoter-only reporter construct (see the legend of Fig. 1 for details).