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. 2024 Mar 7;14:5682. doi: 10.1038/s41598-024-55950-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a–i): Photomicrographs of male albino rat’s splenic sections (X 200 = 100 μm). (a–c): Photomicrographs of rat spleen stained with H&E. (d–f): Photomicrographs of rat spleen stained with Masson’s trichrome stain. (g–i): Photomicrographs of rat spleen prepared for Cd4 receptors immune-staining. Red pulp (RP), White pulp (WP), Marginal zone (M), Central artery (arrow head), Slightly congestion of the blood sinusoids of the red pulp (), Thickened trabecula with large amount of collagen fibers (), Markedly congested blood vessels (thick arrow), Positive expression of CD4 protein (↑), H&E: haematoxylin and eosin, SOV: Sofosbuvir; Ri: Ribavirin.

Inline graphic — (a–i): Photomicrographs of male albino rat’s splenic sections (X 200 = 100 μm). (a–c): Photomicrographs of rat spleen stained with H&E. (d–f): Photomicrographs of rat spleen stained with Masson’s trichrome stain. (g–i): Photomicrographs of rat spleen prepared for Cd4 receptors immune-staining. Red pulp (RP), White pulp (WP), Marginal zone (M), Central artery (arrow head), Slightly congestion of the blood sinusoids of the red pulp (), Thickened trabecula with large amount of collagen fibers (), Markedly congested blood vessels (thick arrow), Positive expression of CD4 protein (↑), H&E: haematoxylin and eosin, SOV: Sofosbuvir; Ri: Ribavirin.