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. 2024 Mar 7;10:26. doi: 10.1038/s41540-024-00353-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative workflow of image processing. The original image was taken with permission from the work²² and shows that the MDA-MB-231 cell nucleus in red is located in a chamber. Scale bar, 10 µm. b Morphological analysis of the sample in the polar coordinate system (PCS). The green and orange bars represent probability density functions (PDFs) of radial and angular displacements, respectively. c Derivation of the CME based on PDFs and Shannon entropy.