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ELISA with purified rCor a 1 isoallergens and sera of tree pollen allergic patients.
The values represent the ratio of the measurements at 450/620 nm normalized to the 450/620 ratio of the negative control (horse serum). Serum dilution 1:10. Two technical replicates were performed.
Light yellow: 1–1.5, yellow: 1.5–5, orange: 5–10, red: 10–20, purple: > 20.
