TABLE 2.
Comparison reference ranges (BD Biosciences IMK Simultest Lymphocyte manual) and original (100 µL) vs modified (25 µL) methodologies as percentages of total gated lymphocytesa
| 100 µL | 25 µL | 95% Reference range | |
|---|---|---|---|
| CD3+ (T) cell, % | 62.1 | 60.9 | 72 (59-85) |
| CD3+CD4+ (T-helper) cell, % | 35.1 | 35.1 | 46 (31-61) |
| CD3+CD8+ (cytotoxic) T cell, % | 29.7 | 23.8 | 25 (11-38) |
| CD4+/CD8+ cell ratio, % | 1.8 | 1.5 | 1.9 (0.9-3.6) |
| CD19+ (B) cell, % | 8.59 | 10.6 | 13 (6.4-23) |
| CD16+ 56+ (NK) cell, % | 9.26 | 6.61 | 14 (5.6-31) |
aSamples taken from 1 participant. Reference ranges obtained from BD Biosciences IMK manual.