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Determination of the accuracy of droplet digital polymerase chain reaction (ddPCR). A, Wild-type and mutant control plasmids were mixed in various proportions, and the m.3243A>G heteroplasmy levels were measured using ddPCR. Theoretical and measured values were plotted thereafter (y = 0.99x – 0.079; R2 = 0.9998). B, The averages of the theoretical and measured values are shown. All experiments were performed in triplicate.
