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Coimmunoprecipitation of hsc70 with Hop/p60 (Hop). hsc70 (0.5 μM) was incubated with Hop/p60 or truncations thereof (0.5 μM each) in either the absence or the presence of GST-Hap46 (2.4 μM). Immunoprecipitations (described in Materials and Methods) were with Penta · His antibody recognizing His-tagged Hop/p60 and fragments thereof. Coprecipitated hsc70 was detected by immunoblotting with antibody N27F3-4.
