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Coimmunoprecipitation of Hop/p60 (Hop) with hsc70. hsc70 (0.5 μM) was incubated with Hop/p60 (0.5 μM) and increasing amounts of GST-Hap46. Immunoprecipitations (described in Materials and Methods) were done with hsc70-specific antiserum K-19. Coprecipitated Hop/p60 was detected by immunoblotting with antibody F5.
