FIG. 8.

Effects on refolding of denatured luciferase. Reactivation of thermally denatured firefly luciferase (Sigma) was carried out as described previously (13, 39) with hsc70 (1.4 μM), hsp40 (1.2 μM), and 5% rabbit reticulocyte lysate (Promega). Reconstituted activity in controls without further additions was set at 100%. Other proteins were added (4 μM each) as indicated. Experiments were done in triplicate. The averages of three separate experiments are given. Error bars show standard deviations. The results shown in bars 1 to 3 correspond to previous data (13) and are included for comparison.