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. 2024 Feb 14;4(2):100709. doi: 10.1016/j.crmeth.2024.100709

Figure 1.

Figure 1

Atlas-based 8aD tFUS target and resultant sonication sites for all four marmosets

(A) Marmoset atlas-based target (8aD) that was integrated into the MORPHEUS (FUSInstruments) software. Ellipse shows the target volume of opening from the 1.46 MHz transducer—the full width at half maximum of the acoustic pressure distribution at the focus.

(B) Sonication target ellipse overlayed on slice from Marmoset M showing both targeting accuracy and highly focal transduction as evidenced by mCherry florescence. Scale bars: 1 mm (horizontal), 2 mm (vertical). (B1) Zoomed-in view of the extent of viral transduction; labeled cells are present in a section of tissue. Scale bars: 1 mm, 2 mm.

(C–F) Gadolinium-enhanced MRI of Marmosets M (C), S (D), T (E), and N (F) showing successful blood-brain barrier perturbation prior to injection of AAV. Note that Marmoset S had a fast low-angle shot (FLASH) image acquired rather than magnetization-prepared rapid gradient echo (MPRAGE) like the other 3 marmosets.