Figure 4.

Comparison of long-range axonal labeling from an intracortical injection vs. systemic tFUS-mediated AAV delivery

(A) Labeled axons in the hemisphere opposite intracortical injection (source: https://cau-gin.brainminds.riken.jp/brainminds/MTI-R01_0088). Note: left-right is swapped for visualization consistency. Scale bar: 2000 μm; zoomed-in (100 μm).

(B) Labeled axons in hemisphere opposite sonication site in Marmoset M (AAV9 mCherry).

(C) A similar contralateral slice for Marmoset N (AAV2 EGFP).

(D) Axons labeled in the corpus callosum from the same intracortical injection as (A) but with a more posterior slice.

(E) AAV9 labeled axons in Marmoset S from systemic injection.

(F) Same as (E) for Marmoset M. White arrows indicate axons expressing fluorescence.