Figure 5.

Functional verification of STAR-cloned KSHV BACs

(A) Production of infectious virus from iSLK-BAC cells. Virus stocks were prepared by chemical induction of iSLK cells carrying KSHV BACs. SLK cells were infected with 1:2-diluted virus stock, and infected cells expressing GFP were quantified 24 h post-infection by flow cytometry. The gating strategy is shown in Figure S1.

(B) Verification of latent infection following BAC-derived virus infection. SLK cells were infected with KSHV BAC-derived virus and stained 24 h post-infection by immunofluorescence for KSHV LANA. Nuclei were counterstained with DAPI. Fluorescence signals were imaged at 63× magnification.

Mock- and BAC16-infected SLK cells were used as a negative and positive controls, respectively, in (A) and (B). Scale bar: 45 μm.