Table 1.

Some of the most notable recent publications on thermal profiling to date

Year	Authors	Method	Description	Replicates	Temperatures	Reference
2013	Molina et al.	CETSA	Western-blot-based thermal profiling	3	12	Savitski et al.5
2014	Savitski et al.	TPP	Mass spectrometry-based thermal profiling	2	10	Martinez Molina et al.6
2015	Franken et al.	TPP	Protocol for mass spectrometry-based thermal profiling	2	10	Franken et al.8
2019	Gaetani et al.	OnePot	Proposed OnePot approach to pool temperatures and increase replicates	5	10-pooled	Gaetani et al.17
2021	Ruan et al.	mTSA	Proposed DIA-OnePot approach for thermal profiling using 52°F temperature and 5 concentrations	5	1	Ruan et al.31
2022	Xu et al.	DDA-OnePot	Applied DDA-OnePot approach within 45°F–58°F for improved sensitivity over 1D thermal profiling	3	10-pooled	George et al.39
2023	Phaneuf et al.	iMAATSA	Proposed the implementation of CARRIER, field asymetric ion mobility spectrometry (FAIMS), and ΦSDM to increase protein identifications and accuracy of thermal profiling	3	10	Dwivedi and Rose44